1G0Y
IL-1 RECEPTOR TYPE 1 COMPLEXED WITH ANTAGONIST PEPTIDE AF10847
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12-03 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 95.898, 95.898, 208.017 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| Rwork | 0.223 |
| R-free | 0.33200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.910 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.103 | 0.369 |
| Total number of observations | 125320 * | |
| Number of reflections | 11894 | |
| <I/σ(I)> | 21.9 | |
| Completeness [%] | 91.0 | 82.3 |
| Redundancy | 10.5 | 73.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 25% polyethylene glycol 4000, 0.2 M ammonium sulfate, 100 mM sodium acetate pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | IL-1R1 | 3.3 (mg/ml) | |
| 2 | 1 | drop | AF10847 | 1:2 molar ratio | |
| 3 | 1 | reservoir | PEG4000 | 25 (%) | |
| 4 | 1 | reservoir | ammonium sulfate | 0.2 (M) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) |
