1G0X

CRYSTAL STRUCTURE OF THE LIGAND BINDING DOMAIN OF LIR-1 (ILT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-01
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	68.258, 68.258, 129.734
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.770 - 2.100
R-factor	0.214
Rwork	0.214
R-free	0.24400
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.060	0.297
Number of reflections	21555	2112 *
<I/σ(I)>	18.8
Completeness [%]	99.5	99.5
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	22 *	potassium sodium tartarate, tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	potassium sodium tartrate	0.7 (M)
3	1	reservoir	Tris-Cl	0.1 (M)	pH8.5