1G0U
A GATED CHANNEL INTO THE PROTEASOME CORE PARTICLE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-09-14 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.180, 301.100, 144.100 |
| Unit cell angles | 90.00, 113.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.25 * |
| Rwork | 0.250 |
| R-free | 0.30300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.878 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.161 | 0.420 * |
| Total number of observations | 1017653 * | |
| Number of reflections | 378678 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 92.6 | 85.4 |
| Redundancy | 2.7 | 0.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 24 * | 22mM Magnesium acetate, 4% Dimethylsulfoxide, 100mM morpholinoethanesulfonic acid, 11% methylpentanediol, pH 6.7, hanging drop vapour-diffusion, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | magnesium acetate | 22 (mM) | |
| 5 | 1 | reservoir | dimethylsulfoxide | 5 (%(v/v)) | |
| 6 | 1 | reservoir | morpholino-ethane-sulphonic | 100 (mM) | |
| 7 | 1 | reservoir | MPD | 11 (%(v/v)) |
