1G0U

A GATED CHANNEL INTO THE PROTEASOME CORE PARTICLE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-09-14
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	135.180, 301.100, 144.100
Unit cell angles	90.00, 113.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.25 *
Rwork	0.250
R-free	0.30300
RMSD bond length	0.012
RMSD bond angle	1.878
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.161	0.420 *
Total number of observations	1017653 *
Number of reflections	378678
<I/σ(I)>	8.4
Completeness [%]	92.6	85.4
Redundancy	2.7	0.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	24 *	22mM Magnesium acetate, 4% Dimethylsulfoxide, 100mM morpholinoethanesulfonic acid, 11% methylpentanediol, pH 6.7, hanging drop vapour-diffusion, temperature 297K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	EDTA	1 (mM)
4	1	reservoir	magnesium acetate	22 (mM)
5	1	reservoir	dimethylsulfoxide	5 (%(v/v))
6	1	reservoir	morpholino-ethane-sulphonic	100 (mM)
7	1	reservoir	MPD	11 (%(v/v))