1G0Q

CRYSTAL STRUCTURE OF T4 LYSOZYME MUTANT V149I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1994-04-25
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	60.590, 60.590, 96.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
Rwork	0.150
RMSD bond length	0.015
RMSD bond angle	1.900
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.000
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.112
Number of reflections	18113
<I/σ(I)>	12
Completeness [%]	90.0	60
Redundancy	2	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	277	Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol