1G0K

CRYSTAL STRUCTURE OF T4 LYSOZYME MUTANT T152C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	300
Detector technology	AREA DETECTOR
Collection date	1994-10-28
Detector	OTHER
Spacegroup name	P 32 2 1
Unit cell lengths	60.690, 60.690, 96.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.850
Rwork	0.155
RMSD bond length	0.015
RMSD bond angle	1.900
Data reduction software	SDMS
Data scaling software	UCSD-system
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.810
High resolution limit [Å]	1.850 *	1.680
Rmerge	0.048	0.211
Number of reflections	20066
<I/σ(I)>	12.1
Completeness [%]	87.0 *	58.4
Redundancy	2.1	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	277	Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol