1G0J

CRYSTAL STRUCTURE OF T4 LYSOZYME MUTANT T152S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	300
Detector technology	AREA DETECTOR
Collection date	1994-10-30
Detector	OTHER
Spacegroup name	P 32 2 1
Unit cell lengths	60.900, 60.900, 96.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
Rwork	0.161
RMSD bond length	0.017
RMSD bond angle	2.100
Data reduction software	SDMS
Data scaling software	UCSD-system
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.880
High resolution limit [Å]	1.800 *	1.740
Rmerge	0.046	0.175
Number of reflections	19639
<I/σ(I)>	13
Completeness [%]	92.0 *	65.1
Redundancy	2.5	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol