1G0F
SITE-SPECIFIC MUTANT (HIS64 REPLACED WITH ALA) OF HUMAN CARBONIC ANHYDRASE II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-03 |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.484, 41.616, 72.658 |
| Unit cell angles | 90.00, 104.52, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.600 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.19800 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 25.000 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.052 | 0.169 * |
| Total number of observations | 139667 * | |
| Number of reflections | 27019 | 1979 * |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 82.6 | 61.5 * |
| Redundancy | 5.17 | 5.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 4 * | Ammonium Sulfate, Mercury Chloride, Tris HCl, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (M) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | reservoir | ammonium sulfate | 2.3-2.5 (M) | |
| 5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 6 | 1 | reservoir | 1 (mM) |
