1G0C
ALKALINE CELLULASE K CATALYTIC DOMAIN-CELLOBIOSE COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.900, 97.900, 121.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.184 * |
| Rwork | 0.181 |
| R-free | 0.20400 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 * | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.187 |
| Number of reflections | 47889 | 3826 * |
| <I/σ(I)> | 20.1 | |
| Completeness [%] | 89.2 | 72.4 |
| Redundancy | 27.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | Cadmium sulfate hydrate, sodium acetate, HEPES, cellobiose, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (%(w/v)) | |
| 2 | 1 | drop | cadmium sulfate hydrate | 20 (mM) | |
| 3 | 1 | drop | sodium acetate | 0.5 (M) | |
| 4 | 1 | drop | HEPES | 0.1 (M) | |
| 5 | 1 | reservoir | cadmium sulfate hydrate | 40 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 1.0 (M) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) |
