1G0A
CARBONMONOXY LIGANDED BOVINE HEMOGLOBIN PH 8.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-01-15 |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.800, 131.400, 63.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.040 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.023 |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | MERLOT |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.530 | 2.200 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.067 | 0.197 |
| Total number of observations | 304865 * | |
| Number of reflections | 38330 | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 96.2 | 86.4 |
| Redundancy | 7.95 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | sodium TAPS, ammonium sulphate, potassium chloride, polyethylene glycol 3350, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | bovine carbonmonoxyhemoglobin | 4 (%) | |
| 2 | 1 | reservoir | TAPS | 100 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 50 (mM) | |
| 4 | 1 | reservoir | potassium chloride | 200 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 19 (%) |
