1G08
CARBONMONOXY LIGANDED BOVINE HEMOGLOBIN PH 5.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1991-09-01 |
Detector | SDMS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.400, 110.900, 66.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.196 |
Rwork | 0.196 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.023 |
Data reduction software | SDMS |
Data scaling software | SDMS |
Phasing software | MERLOT |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.690 | 2.030 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.058 | 0.232 |
Total number of observations | 334873 * | |
Number of reflections | 45723 | |
<I/σ(I)> | 29.45 | |
Completeness [%] | 96.8 | 85.3 |
Redundancy | 7.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 5 | 296 | sodium cacodylate, sodium dithionite, polyethylene glycol 3350, pH 5.0, Batch, temperature 296K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | bovine carbonmonoxyhemoglobin | 2 (%) | |
2 | 1 | 1 | sodium cacodylate | 300 (mM) | |
3 | 1 | 1 | sodium dithionite | 2 (mM) | |
4 | 1 | 1 | Dow PEG3350 | 11.5 (%) |