1FZF
CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN WITH THE PEPTIDE LIGAND GLY-HIS-ARG-PRO-AMIDE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 297 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.800, 149.400, 234.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fzc |
| RMSD bond length | 0.018 |
| RMSD bond angle | 3.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.119 |
| Total number of observations | 232395 * |
| Number of reflections | 49458 |
| Completeness [%] | 88.5 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 * | pH 7.8 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | GHRPam | 10 (mM) | |
| 3 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 4 | 1 | reservoir | 10 (mM) | or 20 mM | |
| 5 | 1 | reservoir | PEG3350 | 12 (%) | |
| 6 | 1 | reservoir | sodium azide | 2 (mM) |
