1FZF

CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN WITH THE PEPTIDE LIGAND GLY-HIS-ARG-PRO-AMIDE

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1997-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	54.800, 149.400, 234.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.233
Rwork	0.233
R-free	0.30200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fzc
RMSD bond length	0.018
RMSD bond angle	3.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Rmerge	0.119
Total number of observations	232395 *
Number of reflections	49458
Completeness [%]	88.5
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7 *		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	GHRPam	10 (mM)
3	1	reservoir	Tris-HCl	50 (mM)
4	1	reservoir		10 (mM)	or 20 mM
5	1	reservoir	PEG3350	12 (%)
6	1	reservoir	sodium azide	2 (mM)