1FZC
CRYSTAL STRUCTURE OF FRAGMENT DOUBLE-D FROM HUMAN FIBRIN WITH TWO DIFFERENT BOUND LIGANDS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-08 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.443, 95.600, 113.641 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.22 * |
| Rwork | 0.220 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FIBRINOGEN FRAGMENT D |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.521 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.093 |
| Total number of observations | 376448 * |
| Number of reflections | 81010 |
| Completeness [%] | 94.5 |
| Redundancy | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7 | 12% PEG 3350, 50 MM TRIS PH 7.0, 5 MM CALCIUM CHLORIDE, 1MM SODIUM AZIDE, 5MM GLY-PRO-ARG-PRO-AMIDE, 5MM GLY-HIS-ARG-PRO-AMIDE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | double-D | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | GPRPam | 5 (mM) | |
| 5 | 1 | drop | GHRPam | 5 (mM) | |
| 6 | 1 | reservoir | Tris | 50 (mM) | |
| 7 | 1 | reservoir | 10 (mM) | ||
| 8 | 1 | PEG3350 | 12 (%) | ||
| 9 | 1 | reservoir | sodium azide | 1 (mM) |
