1FZA
CRYSTAL STRUCTURE OF FIBRINOGEN FRAGMENT D
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 297 |
| Collection date | 1996-02 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.720, 48.080, 167.560 |
| Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.36300 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.630 |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.145 |
| Total number of observations | 318238 * |
| Number of reflections | 35552 |
| Completeness [%] | 87.1 |
| Redundancy | 8.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | SYMMETRY OPERATIONS FOR NON-STANDARD SETTING: X, Y, Z -X, Y+1/2, -Z |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 16-19 (%) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | 2 (mM) | ||
| 4 | 1 | reservoir | 50-133 (mM) |
