1FYU
Crystal structure of erythrina corallodendron lectin in hexagonal crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09-04 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 65 |
| Unit cell lengths | 135.850, 135.850, 82.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.160 - 2.600 |
| R-factor | 0.173 |
| Rwork | 0.173 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMEN |
| Starting model (for MR) | 1LTE.PDB |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.160 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 26125 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 97.3 | 59.9 |
| Redundancy | 8.50 * | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | Elgavish, S., (1998) J. Mol. Biol., 277, 917. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | MPD | 15 (%(v/v)) | |
| 3 | 1 | drop | HEPES | 50 (mM) | |
| 4 | 1 | drop | sodium azide | 0.02 (%(w/v)) | |
| 5 | 1 | reservoir | MPD | 30 (%) | |
| 6 | 1 | reservoir | HEPES | 100 (mM) | |
| 7 | 1 | reservoir | sodium azide | 0.02 (%) |
