1FYN

PHOSPHOTRANSFERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	I 2 2 2
Unit cell lengths	42.000, 43.300, 65.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.300
R-factor	0.171 *
Rwork	0.234
RMSD bond length	0.011 *
RMSD bond angle	1.610
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.088 *
Completeness [%]	88.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.2 *		protein solution is mixed with lyophilized 3BP-2 peptide at a 1:1 molar ratio and subsequently mixed at 1:1(v:v) to reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mM)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop		270 (mM)
4	1	reservoir	sodium citrate	90 (mM)
5	1	reservoir	Bis-Tris propane	90 (mM)
6	1	reservoir	dithiothreitol	0.9 (mM)
7	1	reservoir	EDTA	0.9 (mM)
8	1	reservoir		0.9 (mM)
9	1	reservoir	ammonium sulfate	38-42 (%sat)