1FYN
PHOSPHOTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 42.000, 43.300, 65.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 * - 2.300 |
R-factor | 0.171 * |
Rwork | 0.234 |
RMSD bond length | 0.011 * |
RMSD bond angle | 1.610 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.300 * |
Rmerge | 0.088 * |
Completeness [%] | 88.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.2 * | protein solution is mixed with lyophilized 3BP-2 peptide at a 1:1 molar ratio and subsequently mixed at 1:1(v:v) to reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mM) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | drop | 270 (mM) | ||
4 | 1 | reservoir | sodium citrate | 90 (mM) | |
5 | 1 | reservoir | Bis-Tris propane | 90 (mM) | |
6 | 1 | reservoir | dithiothreitol | 0.9 (mM) | |
7 | 1 | reservoir | EDTA | 0.9 (mM) | |
8 | 1 | reservoir | 0.9 (mM) | ||
9 | 1 | reservoir | ammonium sulfate | 38-42 (%sat) |