1FYK
SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA THAT IS TRANSLATIONALLY DISORDERED
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1995-01-01 |
Detector | FUJI |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.150, 32.990, 42.570 |
Unit cell angles | 90.00, 109.90, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.209 * |
Rwork | 0.209 |
R-free | 0.27800 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.593 |
Data reduction software | DENZO |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.550 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.050 | 0.113 |
Number of reflections | 4940 | |
<I/σ(I)> | 10.3 | |
Completeness [%] | 98.2 | 89.2 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 * | 18 * | PEG 4000, Cacodylate, Ammonium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | Cacodylate | ||
3 | 1 | 1 | Ammonium Acetate |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG4000 | 25-30 (%) | |
2 | 1 | reservoir | cacodylate | 50 (mM) | |
3 | 1 | reservoir | ammonium acetate | 275 (mM) | |
4 | 1 | drop | protein | 0.91 (mM) | |
5 | 1 | drop | DNA | 0.69 (mM) | |
6 | 1 | drop | Tris | 10 (mM) | |
7 | 1 | drop | 50 (mM) | ||
8 | 1 | drop | ammonium acetate | 400 (mM) |