1FYK

SERENDIPITOUS CRYSTAL STRUCTURE CONTAINING THE HEAT SHOCK TRANSCRIPTION FACTOR'S DNA BINDING DOMAIN AND COGNATE DNA THAT IS TRANSLATIONALLY DISORDERED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	FUJI
Spacegroup name	C 1 2 1
Unit cell lengths	103.150, 32.990, 42.570
Unit cell angles	90.00, 109.90, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.209 *
Rwork	0.209
R-free	0.27800
RMSD bond length	0.007
RMSD bond angle	1.593
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.550
High resolution limit [Å]	2.500	2.500
Rmerge	0.050	0.113
Number of reflections	4940
<I/σ(I)>	10.3
Completeness [%]	98.2	89.2
Redundancy	3.7	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *	18 *	PEG 4000, Cacodylate, Ammonium Acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 4000
2	1	1	Cacodylate
3	1	1	Ammonium Acetate

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	25-30 (%)
2	1	reservoir	cacodylate	50 (mM)
3	1	reservoir	ammonium acetate	275 (mM)
4	1	drop	protein	0.91 (mM)
5	1	drop	DNA	0.69 (mM)
6	1	drop	Tris	10 (mM)
7	1	drop		50 (mM)
8	1	drop	ammonium acetate	400 (mM)