1FY7
CRYSTAL STRUCTURE OF YEAST ESA1 HISTONE ACETYLTRANSFERASE DOMAIN COMPLEXED WITH COENZYME A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2000-03-26 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 181.500, 181.500, 181.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.216 |
Rwork | 0.219 |
R-free | 0.23600 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.222 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.063 | 0.272 |
Total number of observations | 1241495 * | |
Number of reflections | 34558 | |
<I/σ(I)> | 48.6 | |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 35.9 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 20 * | amonium phosphate, cacodylate,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | CoA | 0.5 (mM) | |
3 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.7 (M) |