1FY6
AQUIFEX AEOLICUS KDO8P SYNTHASE IN COMPLEX WITH CADMIUM AND A5P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.671, 84.671, 160.036 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.000 * - 1.890 |
Rwork | 0.219 |
R-free | 0.25700 |
RMSD bond length | 0.006 |
RMSD bond angle | 22.900 * |
Data reduction software | SIEMENS |
Data scaling software | SAINT |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.000 * | 2.010 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.080 | 0.258 |
Total number of observations | 381111 * | |
Number of reflections | 45480 | |
<I/σ(I)> | 29.2 | |
Completeness [%] | 84.7 | 65.9 |
Redundancy | 7.4 | 2.43 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 4 * | drop contains protein and reservoir solution in a 1:1 ratio * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 30 (mg/ml) | |
2 | 1 | reservoir | sodium acetate | 100 (mM) | |
3 | 1 | reservoir | PEG4000 | 5-6 (%) |