1FY4
FUSARIUM OXYSPORUM TRYPSIN AT ATOMIC RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, Hamburg |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-03 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 33.132, 36.774, 39.639 |
Unit cell angles | 102.24, 104.75, 102.85 |
Refinement procedure
Resolution | 20.000 - 0.810 |
R-factor | 0.108 |
Rwork | 0.108 |
RMSD bond length | 0.016 |
RMSD bond angle | 0.037 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 0.820 |
High resolution limit [Å] | 0.810 | 0.810 |
Rmerge | 0.038 | 0.027 |
Total number of observations | 1184962 * | |
Number of reflections | 159467 * | |
<I/σ(I)> | 3.1 | |
Completeness [%] | 92.8 | 88.4 |
Redundancy | 7.4 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | sodium sulphate, sodium citrate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (M) | |
2 | 1 | drop | 3,3-dimethyl glutaric acid | 10 (mM) | |
3 | 1 | drop | 2 (mM) | ||
4 | 1 | drop | 100 (mM) | ||
5 | 1 | reservoir | 1.4 (M) | ||
6 | 1 | reservoir | sodium citrate | 0.1 (M) | |
7 | 1 | reservoir | 2-propanol | 0-5 (%) |