1FXW
CRYSTAL STRUCTURE OF THE RECOMBINANT ALPHA1/ALPHA2 CATALYTIC HETERODIMER OF BOVINE BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-07-06 |
Detector | CUSTOM-MADE |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.519, 80.848, 116.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.218 * |
Rwork | 0.218 |
R-free | 0.27700 * |
RMSD bond length | 0.009 * |
RMSD bond angle | 0.023 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 |
High resolution limit [Å] | 2.100 * | 2.100 * |
Rmerge | 0.081 * | 0.335 * |
Total number of observations | 273571 * | |
Number of reflections | 25073 * | |
<I/σ(I)> | 7.63 | |
Completeness [%] | 99.1 * | 99.4 * |
Redundancy | 3.83 | 3.47 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.4 | 298 | 17% PEG-MME 2000, 100 mM Na acetate, pH 6.4, 10 mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG2000 MME | 17 (%) | |
2 | 1 | 1 | sodium acetate | 100 (mM) | |
3 | 1 | 1 | 10 (mM) | ||
4 | 1 | 1 | protein | 12 (mg/ml) |