1FXA

CRYSTALLIZATION AND STRUCTURE DETERMINATION TO 2.5-ANGSTROMS RESOLUTION OF THE OXIDIZED [2FE-2S] FERREDOXIN ISOLATED FROM ANABAENA 7120

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	37.420, 38.120, 147.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.187
RMSD bond length	0.014
RMSD bond angle	24.775 *
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.045 *
Total number of observations	27168 *
Number of reflections	7672 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	2.6 (M)
2	1	drop	potassium succinate	50 (mM)
3	1	drop	MPD	1 (%)