1FX2
STRUCTURAL ANALYSIS OF ADENYLATE CYCLASES FROM TRYPANOSOMA BRUCEI IN THEIR MONOMERIC STATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-08 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.600, 60.070, 78.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.460 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.20500 |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.480 |
| High resolution limit [Å] | 1.460 | 1.440 |
| Rmerge | 0.082 | 0.283 |
| Total number of observations | 498167 * | |
| Number of reflections | 40911 | |
| <I/σ(I)> | 24.7 | |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 291 | 20 mM Tris/HCl, 1.8-1.9 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
| 4 | 1 | reservoir | 0.2 (M) |
