1FX2
STRUCTURAL ANALYSIS OF ADENYLATE CYCLASES FROM TRYPANOSOMA BRUCEI IN THEIR MONOMERIC STATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-12-08 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.600, 60.070, 78.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.460 |
R-factor | 0.178 |
Rwork | 0.178 |
R-free | 0.20500 |
RMSD bond length | 0.031 |
RMSD bond angle | 2.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.480 |
High resolution limit [Å] | 1.460 | 1.440 |
Rmerge | 0.082 | 0.283 |
Total number of observations | 498167 * | |
Number of reflections | 40911 | |
<I/σ(I)> | 24.7 | |
Completeness [%] | 98.7 | 98.9 |
Redundancy | 3 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 * | 291 | 20 mM Tris/HCl, 1.8-1.9 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 18K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 6 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 5 (mM) | |
3 | 1 | reservoir | PEG4000 | 25 (%(w/v)) | |
4 | 1 | reservoir | 0.2 (M) |