1FX0

Crystal structure of the chloroplast F1-ATPase from spinach

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-14
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	147.700, 147.700, 385.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 3.200
R-factor	0.319 *
Rwork	0.319
R-free	0.35000
RMSD bond length	0.005
RMSD bond angle	1.354
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.087	0.288
Total number of observations	231238 *
Number of reflections	23050
<I/σ(I)>	6.4
Completeness [%]	86.2	76
Redundancy	3.9	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	20 *	ammonium sulfate, HEPES, Na-azide, pH 7.5, Microbatch, temperature 25K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	HEPES	25 (mM)
2	1	1	dithiothreitol	1 (mM)
3	1	1	PMSF	0.002 (%(w/v))
4	1	1		0.01 (%(w/v))
5	1	1	ammonium sulfate	1.5 (M)