1FWZ
GLU20ALA DTXR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-01-10 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 63.700, 63.700, 106.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.28500 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.084 | 0.400 |
| Number of reflections | 9634 | |
| <I/σ(I)> | 17.4 | |
| Completeness [%] | 81.0 | 74 |
| Redundancy | 6.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 298 | 1.6-2.0 ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris | 10 (mM) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | protein | 2.2 (mg/ml) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) | |
| 6 | 1 | reservoir | glycerol | 5 (%) |
