1FWV

CRYSTAL STRUCTURE OF THE CYSTEINE-RICH DOMAIN OF MANNOSE RECEPTOR COMPLEXED WITH 3-SO4-LEWIS(A)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2000-02-21
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	39.608, 41.158, 99.646
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.200
R-factor	0.208 *
Rwork	0.208
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.034	0.085 *
Total number of observations	64935 *
Number of reflections	8538
<I/σ(I)>	31
Completeness [%]	96.5	89.1
Redundancy	7.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4 *	4 *	PEG 8000, (NH4)2SO4, pH 6.5-7.5 , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop		10 (mM)
4	1	reservoir	ammonium sulfate	0.2 (M)
5	1	reservoir	PEG8000	30 (%(w/v))