Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-22 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 37.802, 37.802, 99.779 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.218 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cll |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.400 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.367 * |
| Number of reflections | 8839 | |
| <I/σ(I)> | 11.9 | 1.95 |
| Completeness [%] | 95.4 | 99.3 * |
| Redundancy | 3.7 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 4000, calcium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 4000, calcium chloride, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 88 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 15 (%) | |
| 3 | 1 | reservoir | sodium acetate | 40 (mM) | |
| 4 | 1 | reservoir | 70 (mM) |
