1FW3
OUTER MEMBRANE PHOSPHOLIPASE A FROM ESCHERICHIA COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.540, 84.970, 95.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.900 - 2.800 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.28100 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.900 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.121 | 0.286 |
| Total number of observations | 53984 * | |
| Number of reflections | 14611 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 81.0 | 53.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 20 * | b-OG, Tris, Potassium Chloride, Ammonium phosphate, pH 6.6, VAPOR DIFFUSION, HANGING DROP at 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | beta-OG | 1.95 (%(w/v)) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | Tris | 2 (mM) | |
| 5 | 1 | reservoir | PEG400 | 12-17 (%(v/v)) | can be replaced by 0.25M ammonium phosphate |
