1FVV
THE STRUCTURE OF CDK2/CYCLIN A IN COMPLEX WITH AN OXINDOLE INHIBITOR
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 62 2 2 |
| 格子定数 [Å] | 184.952, 184.952, 212.832 |
| 格子定数 [度] | 90.00, 90.00, 120.00 |
精密化法
| 残基 | 20.000 - 2.800 |
| R因子 | 0.21 |
| Rwork | 0.260 |
| R-free | 0.26000 |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.009 * |
| 結合角の平均二乗偏差(RMSD) [度] | 1.390 * |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Quality characteristics
| Overall | Outer shell | |
| 分解能 [Å] (低) | 20.000 | 2.980 |
| 分解能 [Å] (高) | 2.800 | 2.800 |
| Rmerge_l_obs | 0.070 | 0.290 |
| Total number of observations | 541713 * | |
| 独立反射数 | 50936 | |
| <I/σ(I)> | 36 | |
| 完全性 [%] | 95.9 | 90.2 |
| 冗長性 | 10 | 9 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | Shewchuk L., (2000) J. Med. Chem., 43, 133. * |
文献の結晶化試薬
| ID | 結晶ID | 溶液 | 試薬名 | 濃度 (単位) (単位) | 詳細 |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | reservoir | PEG3340 | 10 (%) |
