1FVR
TIE2 KINASE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 118 |
| Detector technology | CCD |
| Collection date | 2000-01-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.494, 92.887, 70.798 |
| Unit cell angles | 90.00, 108.93, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.225 |
| R-free | 0.22500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.150 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.075 | 0.200 |
| Number of reflections | 39864 | |
| <I/σ(I)> | 12 | |
| Completeness [%] | 96.4 | 95 * |
| Redundancy | 4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 2.5% PEG12000, 2.5% glycerol, 100mM Hepes, 10mM spermidine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | HEPES | 20 (mM) | |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | reservoir | PEG12000 | 2.5 (%) | |
| 6 | 1 | reservoir | glycerol | 2.5 (%) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | spermidine | 10 (mM) |
