1FVO
CRYSTAL STRUCTURE OF HUMAN ORNITHINE TRANSCARBAMYLASE COMPLEXED WITH CARBAMOYL PHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | I 2 3 |
Unit cell lengths | 203.426, 203.426, 203.426 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.990 - 2.600 |
R-factor | 0.202 |
Rwork | 0.202 |
R-free | 0.23600 |
RMSD bond length | 0.006 |
RMSD bond angle | 23.100 * |
Data reduction software | DENZO |
Data scaling software | CCP4 ((TRUNCATE)) |
Phasing software | GLRF |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.760 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.108 | 0.029 |
Total number of observations | 300506 * | |
Number of reflections | 42459 | |
<I/σ(I)> | 6.8 | |
Completeness [%] | 98.7 | 94.1 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | 291 | Mes, PEG 8000, pH 6.5, EVAPORATION, temperature 291.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | Mes | 0.1 (M) | |
2 | 1 | reservoir | PEG8000 | 16 (%(w/v)) |