1FVI
CRYSTAL STRUCTURE OF CHLORELLA VIRUS DNA LIGASE-ADENYLATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.170, 58.440, 101.790 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.000 |
R-factor | 0.185 * |
Rwork | 0.190 |
R-free | 0.27000 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.548 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.000 * |
Rmerge | 0.045 * |
Completeness [%] | 98.6 * |
Redundancy | 8.8 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | 22 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-HCl | 50 (mM) | |
2 | 1 | drop | dithiothreitol | 5 (mM) | |
3 | 1 | drop | protein | 18 (mg/ml) | |
4 | 1 | drop | 100 (mM) | ||
5 | 1 | reservoir | Tris-HCl | 50 (mM) | |
6 | 1 | reservoir | dithiothreitol | 5 (mM) | |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | ammonium sulfate | 1.8-2.0 (M) |