1FVH
CRYSTAL STRUCTURE ANALYSIS OF NEURONAL SEC1 FROM THE SQUID L. PEALEI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-03-07 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.125, 123.183, 63.891 |
Unit cell angles | 90.00, 110.49, 90.00 |
Refinement procedure
Resolution | 14.910 - 2.800 |
R-factor | 0.234 * |
Rwork | 0.234 |
R-free | 0.28300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1epu |
RMSD bond length | 0.008 |
RMSD bond angle | 22.699 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.054 | 0.287 |
Number of reflections | 16849 | |
<I/σ(I)> | 20.1 | 2.5 |
Completeness [%] | 94.8 | 72.6 |
Redundancy | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 5.6 * | 277 | Bracher, A., (2000) Acta Crystallogr., Sect.D, 56, 501. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG1000 | 15 (%(w/v)) | |
2 | 1 | reservoir | ammonium sulfate | 0.4 (M) | |
3 | 1 | reservoir | sodium citrate | 0.1 (M) | |
4 | 1 | reservoir | dithiothreitol | 20 (mM) | |
5 | 1 | reservoir | 10 (mM) |