1FV9

Crystal structure of human microurokinase in complex with 2-amino-5-hydroxy-benzimidazole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	160
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	55.160, 53.000, 82.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.248
Rwork	0.248
RMSD bond length	0.026
RMSD bond angle	28.900 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall
High resolution limit [Å]	3.000 *
Rmerge	0.101 *
Completeness [%]	99.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4 *	18-24 *	0.15M LiSo4, 20% PEG 4000, succinate buffer, VAPOR DIFFUSION, HANGING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	citrate	10 (mM)
3	1	drop	epsilon-amino caproic acid rho-carbethoxyphenyl ester chloride	3 (mM)
4	1	drop	DMSO cosolvent	1 (%)
5	1	reservoir		0.15 (M)
6	1	reservoir	PEG4000	20 (%)
7	1	reservoir	succinate