1FUQ

FUMARASE WITH BOUND 3-TRIMETHYLSILYLSUCCINIC ACID

Experimental procedure

Temperature [K]	294
Detector technology	AREA DETECTOR
Collection date	1995
Detector	SIEMENS
Spacegroup name	C 2 2 21
Unit cell lengths	104.000, 219.900, 86.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.187
Rwork	0.187
R-free	0.23300
RMSD bond length	0.007
RMSD bond angle	21.150 *
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.078 *
Number of reflections	66809 *
Completeness [%]	94.2 *
Redundancy	4.04 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	citrate	300 (mM)
2	1	reservoir	PEG4000	14 (%)