1FUF
CRYSTAL STRUCTURE OF A 14BP RNA OLIGONUCLEOTIDE CONTAINING DOUBLE UU BULGES: A NOVEL INTRAMOLECULAR U*(AU) BASE TRIPLE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-04-13 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.060, 29.100, 52.570 |
| Unit cell angles | 90.00, 120.69, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.199 * |
| Rwork | 0.199 |
| R-free | 0.22700 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.910 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.052 * | 0.213 |
| Number of reflections | 16802 * | |
| <I/σ(I)> | 21.7 | |
| Completeness [%] | 98.6 * | 88.5 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | cacodylate, magnesium chloride, spermine tetrachloride, MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 25.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | CACODYLATE | ||
| 2 | 1 | 1 | MAGNESIUM CHLORIDE | ||
| 3 | 1 | 1 | SPERMINE TETRACHLORIDE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | RNA | 1 (mM) | double strand |
| 2 | 1 | drop | spermine tetrahydrochloride | 10 (mM) | |
| 3 | 1 | drop | sodium cacodylate | 40 (mM) | |
| 4 | 1 | drop | 10 (%(v/v)) | ||
| 5 | 1 | drop | 20 (mM) | ||
| 6 | 1 | reservoir | MPD | 40 (%) |
