1FU0
CRYSTAL STRUCTURE ANALYSIS OF THE PHOSPHO-SERINE 46 HPR FROM ENTEROCOCCUS FAECALIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 290 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-12-14 |
| Detector | WEISSENBERG |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 25.520, 43.200, 60.750 |
| Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.23900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 22.930 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.041 | 0.110 |
| Total number of observations | 42471 * | |
| Number of reflections | 10043 | 447 * |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 95.1 | 84.3 |
| Redundancy | 4.23 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 287 | used seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | sodium potassium phosphate | 1.4 (M) | |
| 3 | 1 | reservoir | sodium potassium phosphate | 2.8 (M) |
