1FTO
CRYSTAL STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2J) IN THE APO STATE AT 2.0 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1999-06-13 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.170, 91.550, 98.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.224 * |
Rwork | 0.224 |
R-free | 0.28700 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.586 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.036 | 0.148 |
Total number of observations | 153854 * | |
Number of reflections | 37087 | |
<I/σ(I)> | 40.4 | |
Completeness [%] | 95.9 | 73.7 |
Redundancy | 4.15 | 2.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | 22% PEG 8000 0.3 M ammonium sulfate 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | protein | 3 (mM) | |
5 | 1 | drop | glycerol | 10 (%(v/v)) | |
6 | 1 | reservoir | PEG8000 | 19-24 (%) | |
7 | 1 | reservoir | ammonium sulfate | 0.25-0.4 (M) | |
8 | 1 | reservoir | 0.1 (M) |