1FTL
CRYSTAL STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2J) IN COMPLEX WITH THE ANTAGONIST DNQX AT 1.8 A RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1999-10-20 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.153, 93.703, 97.369 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.219 * |
Rwork | 0.219 |
R-free | 0.27200 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.421 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.053 | 0.084 |
Total number of observations | 298773 * | |
Number of reflections | 50796 | |
<I/σ(I)> | 40.6 | |
Completeness [%] | 95.0 | 83.8 |
Redundancy | 5.88 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | 19% PEG 4000 0.3 M ammonium sulfate 0.1 M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | DNQX | 3 (mM) | |
5 | 1 | reservoir | PEG4000 | 17-22 (%) | |
6 | 1 | reservoir | ammonium sulfate | 0.25-0.4 (M) | |
7 | 1 | reservoir | 0.1 (M) |