1FTJ
CRYSTAL STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2J) IN COMPLEX WITH GLUTAMATE AT 1.9 RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-20 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 114.002, 163.363, 47.396 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.226 * |
| Rwork | 0.226 |
| R-free | 0.26600 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.517 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.042 | 0.199 |
| Total number of observations | 391030 * | |
| Number of reflections | 69601 | |
| <I/σ(I)> | 24 | |
| Completeness [%] | 98.3 | 87.8 |
| Redundancy | 5.62 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 277 | 0.1 M Cacodylate 15% PEG 8000 0.2 M Zinc Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 10 (mM) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | L-glutamate | 10 (mM) | |
| 5 | 1 | reservoir | PEG8000 | 13-18 (%) | |
| 6 | 1 | reservoir | 0.1-0.25 (M) | ||
| 7 | 1 | reservoir | cacodylate | 0.1 (M) |
