1FTH
CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE ACYL CARRIER PROTEIN SYNTHASE (3'5'-ADP COMPLEX)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 120.188, 62.273, 51.673 |
| Unit cell angles | 90.00, 98.75, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.238 * |
| Rwork | 0.238 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fte |
| RMSD bond length | 0.009 |
| RMSD bond angle | 21.979 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | 0.268 |
| Total number of observations | 304946 * | |
| Number of reflections | 29541 | |
| <I/σ(I)> | 31.1 | 3.1 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.1 * | 294 | VAPOR DIFFUSION, HANGING DROP, pH 4.5 at 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 14 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 20 (mM) | |
| 5 | 1 | reservoir | PEG4000 | 8-15 (%) | |
| 6 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 7 | 1 | reservoir | citrate | 100 (mM) |
