1FTG

STRUCTURE OF APOFLAVODOXIN: CLOSURE OF A TYROSINE/TRYPTOPHAN AROMATIC GATE LEADS TO A COMPACT FOLD

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-08-25
Spacegroup name	P 21 21 21
Unit cell lengths	55.480, 38.600, 60.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.182
Rwork	0.182
RMSD bond length	0.009
RMSD bond angle	26.000 *
Data reduction software	MOSFLM
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.000 *
Rmerge	0.074
Total number of observations	56317 *
Number of reflections	8437
Completeness [%]	92.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-12 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	reservoir	ammonium sulfate	3.2 (M)
4	1	reservoir	sodium potassium phosphate	0.1 (M)