1FTC

Y13C MUTANT OF AZOTOBACTER VINELANDII FDI

Experimental procedure

Detector technology	AREA DETECTOR
Detector	XENTRONICS
Spacegroup name	P 1 21 1
Unit cell lengths	39.300, 73.700, 45.100
Unit cell angles	90.00, 98.40, 90.00

Refinement procedure

Resolution	8.000 - 2.350
R-factor	0.216
Rwork	0.216
RMSD bond length	0.016
RMSD bond angle	1.490 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.350 *	2.350 *
Rmerge	0.067
Total number of observations	25133 *
Number of reflections	9115 *
Completeness [%]	85.2 *	32.9 *
Redundancy	2.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.5 (mg/ml)
2	1	drop	Tris-HCl	12.5 (mM)
3	1	drop	sodium citrate	0.63 (M)
4	1	drop	beta-mercaptoethanol	0.25 (mM)
5	1	reservoir	sodium citrate	1.26 (M)
6	1	reservoir	beta-mercaptoethanol	0.5 (mM)