1FT9
STRUCTURE OF THE REDUCED (FEII) CO-SENSING PROTEIN FROM R. RUBRUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 1999-05-17 |
Detector | ADSC |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.253, 92.253, 143.723 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.25 |
Rwork | 0.230 |
R-free | 0.28000 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.260 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.800 | 0.400 |
Number of reflections | 21881 | |
<I/σ(I)> | 12 | |
Completeness [%] | 98.7 | 99.1 |
Redundancy | 5 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.5 | 292 | 0.3 M MgCl2, 0.1 M Tris, 20% PEG 8000, pH 8.5, LIQUID DIFFUSION, temperature 292K |
1 | unknown * | 8.5 | 292 | 0.3 M MgCl2, 0.1 M Tris, 20% PEG 8000, pH 8.5, LIQUID DIFFUSION, temperature 292K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 10 (mg/ml) | |
2 | 1 | 1 | Tris-HCl | 0.1 (M) | |
3 | 1 | 1 | 0.6 (M) | ||
4 | 1 | 1 | PEG8000 | 20 (%(v/v)) | |
5 | 1 | 1 | glycerol | 20 (%(v/v)) |