1FT8

CRYSTAL STRUCTURE OF THE RNA-BINDING DOMAIN OF THE MRNA EXPORT FACTOR TAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2000-02-03
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	139.918, 139.918, 206.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.150
R-factor	0.303
Rwork	0.303
R-free	0.30300
RMSD bond length	0.015
RMSD bond angle	1.750
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.250
High resolution limit [Å]	3.150	3.150
Rmerge	0.064	0.280
Total number of observations	227961 *
Number of reflections	35832
<I/σ(I)>	11.6
Completeness [%]	99.6 *	99.9
Redundancy	6.4	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.8	4 *	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)
3	1	reservoir	PEG8000	18 (%(w/v))
4	1	reservoir	EDTA	20 (mM)