1FT7
AAP COMPLEXED WITH L-LEUCINEPHOSPHONIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 4 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 107.000, 107.000, 102.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.200 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.23200 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.153 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.170 |
| Rmerge | 0.134 * | 0.650 |
| Total number of observations | 349668 * | |
| Number of reflections | 21129 * | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 99.9 * | 100 * |
| Redundancy | 6.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 | 293 | KSCN, NaCl, Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | KSCN | 10 (mM) | |
| 4 | 1 | drop | 0.4 (M) | ||
| 5 | 1 | drop | LPA | 4-fold molar excess | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | KSCN | 100 (mM) | |
| 8 | 1 | reservoir | 4.5 (M) |
