1FT5

CRYSTAL STRUCTURE OF THE OXIDIZED STATE OF CYTOCHROME C554 FROM NITROSOMONAS EUROPAEA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	1998-04-23
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	147.892, 147.892, 33.913
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.188 *
Rwork	0.188
R-free	0.21500
RMSD bond length	0.021
RMSD bond angle	1.990
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.174
Total number of observations	109601 *
Number of reflections	35562
<I/σ(I)>	28
Completeness [%]	97.3	86.1
Redundancy	3.08

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.1	22 *	Iverson, T.M., (1998) Nat.Struct.Biol., 5, 1005. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	reservoir	potassium phosphate	62.5 (%(w/v))	pH10.1
3	1	reservoir	potassium phosphate	92.5 (%(w/v))	pH10.1