1FT3
CRYSTAL STRUCTURE OF TRUNCATED RHOGDI K141A MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-12-06 |
Detector | MAR CCD 165 mm |
Spacegroup name | H 3 2 |
Unit cell lengths | 129.610, 129.610, 164.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
Rwork | 0.216 |
R-free | 0.26000 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.440 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.094 | 0.680 * |
Total number of observations | 57299 * | |
Number of reflections | 13249 | |
<I/σ(I)> | 11 | |
Completeness [%] | 99.7 | 87 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.6 | 293 | ammonium sulfate, Na/K tartrate, sodium citrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-15 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 50 (%sat) | |
3 | 1 | reservoir | sodium/potassium tartrate | 200 (mM) | |
4 | 1 | reservoir | sodium citrate | 100 (mM) |