1FSW
AMPC BETA-LACTAMASE FROM E. COLI COMPLEXED WITH INHIBITOR CEPHALOTHINBORONIC ACID
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 5ID-B |
Synchrotron site | APS |
Beamline | 5ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-05-16 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 118.110, 77.829, 97.318 |
Unit cell angles | 90.00, 115.83, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.194 * |
Rwork | 0.194 |
R-free | 0.22700 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.030 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.063 | 0.172 |
Total number of observations | 176832 * | |
Number of reflections | 58505 | |
<I/σ(I)> | 14.2 | |
Completeness [%] | 93.6 | 94.5 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 23 * | used microseeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | potassium phosphate | 1.7 (M) | |
2 | 1 | drop | protein | 0.095 (mM) | |
3 | 1 | drop | DMSO | 2 (%) | |
4 | 1 | drop | potassium phosphate | 1.7 (M) |