1FSU
Crystal Structure of 4-Sulfatase (human)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-09 |
| Detector | RIGAKU |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 107.000, 107.000, 144.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 9999.000 * - 2.500 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.24300 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.590 | |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.089 * | |
| Total number of observations | 194673 * | |
| Number of reflections | 27332 | |
| Completeness [%] | 92.0 | 72.9 |
| Redundancy | 7.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.1 | Ashby, S.J., (1995) Acta Cryst. D, 51, 1082. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 0.025 (M) | |
| 3 | 1 | drop | PEG8000 | 10 (%) | |
| 4 | 1 | reservoir | potassium phosphate | 0.2 (M) | |
| 5 | 1 | reservoir | PEG8000 | 14 (%) |
