1FSO
CRYSTAL STRUCTURE OF TRUNCATED HUMAN RHOGDI QUADRUPLE MUTANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9B |
Synchrotron site | NSLS |
Beamline | X9B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-04-01 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 76.290, 76.290, 88.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
R-factor | 0.218 |
Rwork | 0.217 |
R-free | 0.23200 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.390 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.029 | 0.300 * |
Total number of observations | 83504 * | |
Number of reflections | 20565 | |
<I/σ(I)> | 13.7 | |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 4.1 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6 | 293 | ammonium sulfate and MES buffer, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7-15 (mg/ml) | |
2 | 1 | reservoir | ammonium sulfate | 44-48 (%sat) | |
3 | 1 | reservoir | MES | 100 (mM) |